The Use of CRISPR/cas9, ZFNs, TALENs in Generating Site-Specific Genome Alterations (Methods in Enzymology, Volume 546)

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization scienceContains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease